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Year : 2020  |  Volume : 17  |  Issue : 1  |  Page : 31-36

Entrococcus faecalis elimination in retreatment cases using passive ultrasonic irrigation, manual dynamic activation and photodynamic therapy: a randomized clinical trial

1 Department of Endodontics, Faculty of Dentistry, Zonguldak Bülent Ecevit University, Zonguldak, Turkey
2 Microbiology Laboratory, Erzurum Region Training and Research Hospital, Erzurum, Turkey

Correspondence Address:
Sevinç A. Türker
Department of Endodontics, Faculty of Dentistry, Bülent Ecevit University, Kozlu 67600, Zonguldak
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/tdj.tdj_42_19

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Aim The aim of this in-vivo study was to compare the effect of passive ultrasonic irrigation, manual dynamic activation and photodynamic therapy in reducing Enterococcus faecalis load in retreatment cases by using PCR. Materials and methods Single-rooted, single-canalled 36 teeth with post-treatment apical periodontitis were selected for this study. Teeth were divided into three groups according to the disinfection methods: passive ultrasonic irrigation, manual dynamic activation, and photodynamic therapy (PDT). Microbiological samples were taken after removal of gutta-percha (S1), after preparation of the root canal (S2), and after disinfection method (S3) with sterile paper points. The E. faecalis amounts were measured by using PCR. Results Intragroup analysis revealed that, all groups showed a significant decrease between S1 and S2 (P ≤ 0.05). However, a significant decrease between S2 and S3 was found only with PDT group (P ≤ 0.05). Intergroup analysis revealed that no significant differences were found among disinfection methods for bacterial load of S1, S2 and S3 (P ≥ 0.05). There were no significant differences among disinfection methods in terms of E. faecalis reduction percentages (from S2 to S3) (P ≥ 0.05). Conclusion PDT method significantly enhanced E. faecalis elimination after chemomechanical preparation. All disinfection methods were effective in reducing E. faecalis from infected root canals with no statistical differences.

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