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ORIGINAL ARTICLE
Year : 2019  |  Volume : 16  |  Issue : 3  |  Page : 163-168

Effect of two tissue inhibitors on the antimicrobial activity of chitosan nanoparticles and chlorhexidine


1 Department of Endodontic, Faculty of Dentistry, Tanta University, Tanta, Egypt
2 Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Tanta University, Tanta, Egypt

Correspondence Address:
Muhammad S. Anwar
Department of Endodontic, Faculty of Dentistry, Tanta University, Tanta, Algharbeia
Egypt
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/tdj.tdj_26_19

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Aim: To assess the effect of dentin powder (DB) and human serum albumin (HSA) as tissue inhibitors on the antibacterial activity of chitosan nanoparticles (CSnps) and chlorhexidine (CHX). Materials and methods: The antibacterial effect of CSnps and CHX in presence or absence of inhibitors was tested on planktonic Enterococcus faecalis OG1RF standard strain. Study groups were group 1: DB and CSnps, group 2: DB and CHX, group 3: HSA and CSnps, and group 4: HSA and CHX, group 5: CSnps suspension (positive control for groups 1, 3), group 6: CHX solution (positive control for groups 2, 4), and bacteria suspension as a negative control (group 7). The assigned medications were added to tissue inhibitors for an hour before bacterial suspension addition. After 1 min, 24, and 72 h of bacterial suspension addition a sample was taken. Bacterial culturing was done on brain heart infusion plates at 37°C. The plates were incubated for 24 h and were inspected for growth by naked eye and the colonies the number of surviving bacteria was calculated. Data were collected and the mean and SD of log10 colony forming units was calculated and statistically analyzed using one-way analysis of variance and pairwise comparison between groups was performed whenever significant difference was found. Level of significance was set at P value less than or equal to 0.05. Results: Group 3and group 7 were statistically significant than all tested groups at all times. Also, significant difference between group 4 versus group 2 and group 6 recording P value of 0.048 at 1 min. However, there was no significant difference between groups 1, 2, 4, 5, 6 at 24 and 72 h. Conclusions: HSA had significant inhibitory effect on antibacterial activity of CHX and CSnps than DB at 1 min. At 24 and 72 h, neither HSA nor DB had significant inhibitory effect on antibacterial activity of CHX while HSA had a significant effect on antibacterial activity of CSnps.


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