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ORIGINAL ARTICLE
Year : 2017  |  Volume : 14  |  Issue : 4  |  Page : 208-215

Evaluation of carcinoma-associated fibroblasts in oral squamous cell carcinoma: a potential role for cathepsin K


Department of Oral Pathology, Faculty of Dentistry, Tanta University, Tanta, Egypt

Correspondence Address:
Basant H Abou Zaid
Department of Oral Pathology, Faculty of Dentistry, Tanta University, Medical Campus, Algeysh Street, Tanta
Egypt
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/tdj.tdj_31_17

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Aim The aim of this work was to identify carcinoma-associated fibroblasts (CAFs) in the stroma of oral squamous cell carcinoma (OSCC), through examining α-smooth muscle actin (α-SMA) expression immunohistochemically. Also, to investigate the immunohistochemical expression pattern of cathepsin K (CTSK) in CAFs as well as to assess the expression level of CTSK mRNA in CAFs, by real-time reverse transcription PCR (RT-PCR) technique. Furthermore, to correlate CTSK expression in different histopathological grades of OSCC, in order to identify its potential role in tumor progression and aggressiveness. Materials and methods Paraffin-embedded OSCC specimens were stained immunohistochemically using antibodies against α-SMA and CTSK. At the same time, level of CTSK mRNA expression was evaluated in fibroblasts indirectly cocultured with OSCC cells by means of real-time RT-PCR technique. Results In addition to CTSK coimmunolocalization with α-SMA in stromal CAFs, its expression by OSCC cells was significantly correlated with increased malignant criteria. Stromal CAFs showed upregulated CTSK expression in poorly differentiated OSCC. Real-time RT-PCR results revealed enhanced CTSK mRNA expression by fibroblasts in indirect coculture. Conclusion Collectively, the results propose an evolving role for CTSK in OSCC progression.


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