• Users Online: 296
  • Home
  • Print this page
  • Email this page
Home About us Editorial board Ahead of print Current issue Search Archives Submit article Instructions Subscribe Contacts Login 
Year : 2017  |  Volume : 14  |  Issue : 4  |  Page : 208-215

Evaluation of carcinoma-associated fibroblasts in oral squamous cell carcinoma: a potential role for cathepsin K

Department of Oral Pathology, Faculty of Dentistry, Tanta University, Tanta, Egypt

Correspondence Address:
Basant H Abou Zaid
Department of Oral Pathology, Faculty of Dentistry, Tanta University, Medical Campus, Algeysh Street, Tanta
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/tdj.tdj_31_17

Rights and Permissions

Aim The aim of this work was to identify carcinoma-associated fibroblasts (CAFs) in the stroma of oral squamous cell carcinoma (OSCC), through examining α-smooth muscle actin (α-SMA) expression immunohistochemically. Also, to investigate the immunohistochemical expression pattern of cathepsin K (CTSK) in CAFs as well as to assess the expression level of CTSK mRNA in CAFs, by real-time reverse transcription PCR (RT-PCR) technique. Furthermore, to correlate CTSK expression in different histopathological grades of OSCC, in order to identify its potential role in tumor progression and aggressiveness. Materials and methods Paraffin-embedded OSCC specimens were stained immunohistochemically using antibodies against α-SMA and CTSK. At the same time, level of CTSK mRNA expression was evaluated in fibroblasts indirectly cocultured with OSCC cells by means of real-time RT-PCR technique. Results In addition to CTSK coimmunolocalization with α-SMA in stromal CAFs, its expression by OSCC cells was significantly correlated with increased malignant criteria. Stromal CAFs showed upregulated CTSK expression in poorly differentiated OSCC. Real-time RT-PCR results revealed enhanced CTSK mRNA expression by fibroblasts in indirect coculture. Conclusion Collectively, the results propose an evolving role for CTSK in OSCC progression.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded74    
    Comments [Add]    

Recommend this journal